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Extraction and analysis of high-quality chloroplast DNA with reduced nuclear DNA for medicinal plants
2025-01-15 20:45:07| Spiritual Career Counseling
Research Open access Published: 18 April 2024 Yifan Yu1,2, Xinxin Wang1, Renjun Qu1, Zhen OuYang2, Juan Guo1, Yujun Zhao1 & Luqi Huang1,2 BMC Biotechnology volume24, Articlenumber:20 (2024) Cite this article 1502 Accesses 1 Citations Metrics details AbstractBackgroundObtaining high-quality chloroplast genome sequences requires chloroplast DNA (cpDNA) samples that meet the sequencing requirements. The quality of extracted cpDNA directly impacts the efficiency and accuracy of sequencing analysis. Currently, there are no reported methods for extracting cpDNA from Erigeron breviscapus. Therefore, we developed a suitable method for extracting cpDNA from E. breviscapus and further verified its applicability to other medicinal plants.ResultsWe conducted a comparative analysis of chloroplast isolation and cpDNA extraction using modified high-salt low-pH method, the high-salt method, and the NaOH low-salt method, respectively. Subsequently, the number of cpDNA copies relative to the nuclear DN...
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How to Paint Insulation Foam Board for a Stunning and Durable Finish
2025-01-14 23:45:05| Spiritual Career Counseling
Have you ever looked at your insulation foam board and thought it could use a little color? Whether youre working on a DIY project or sprucing up your space, painting foam board can transform its appearance and enhance its durability.Table of ContentsKey TakeawaysOverview of Insulation Foam BoardPreparing the Surface for PaintingChoosing the Right PaintStep-by-Step Painting ProcessTips for a Successful Paint JobConclusionFrequently Asked QuestionsKey Takeaways Understanding Insulation Foam Board: Recognizes its benefits such as energy efficiency, moisture resistance, and ease of handling for various applications, including building envelopes and HVAC duct insulation.Proper Surface Preparation: Emphasizes the importance of cleaning and repairing foam board before painting to ensure optimal paint adhesion and a smooth finish.Choosing the Right Paint: Highlights suitable paint types like acrylic latex, water-based, and specific spray paints that wont damage foam board, along with consid...
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Shaving Foam Sensory Play Ideas Play of the Wild
2025-01-14 23:45:05| Spiritual Career Counseling
Posted May 17, 2020 by Sabrina2 Comments on Shaving Foam Sensory Play IdeasCreativityShaving foam is a lovely sensory play activity for children, especially in the warmer months! Young children enjoy the foamy, slimy sensation when they squeeze it and rub it. You can also add different things to it or use it in many different ways. Below we will look at five different shaving foam play activities and how they can benefit children.There are many learning benefits to sensory play. The development on new connections in the brain is enhanced when we use and engage our senses (sight, touch, smell, taste, sound or vestibular & proprioception our sense of balance, body position and place in space). The more sensory experiences we have, the better the ability we have to develop pathways in the brain for thought, learning and creativity (Gascoyne, 2016). It can also be an excellent way for children to make marks and practice drawing and writing.What you need for shaving foam sensory playShavi...
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